scripts matlab 2019b Search Results


in house matlab script  (MathWorks Inc)
96
MathWorks Inc in house matlab script
Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house <t>MATLAB</t> script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).
In House Matlab Script, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab software scripts  (MathWorks Inc)
96
MathWorks Inc matlab software scripts
Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house <t>MATLAB</t> script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).
Matlab Software Scripts, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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originpro  (OriginLab corp)
90
OriginLab corp originpro
Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house <t>MATLAB</t> script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).
Originpro, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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em2040 wcd amplitudes  (Kongsberg Maritime Contros GmbH)
90
Kongsberg Maritime Contros GmbH em2040 wcd amplitudes
Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house <t>MATLAB</t> script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).
Em2040 Wcd Amplitudes, supplied by Kongsberg Maritime Contros GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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meg data analysis  (MathWorks Inc)
96
MathWorks Inc meg data analysis
Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house <t>MATLAB</t> script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).
Meg Data Analysis, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10-nm fwhm bandpass filters  (Thorlabs)
90
Thorlabs 10-nm fwhm bandpass filters
Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house <t>MATLAB</t> script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).
10 Nm Fwhm Bandpass Filters, supplied by Thorlabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaris x64 9 3 0  (Oxford Instruments)
99
Oxford Instruments imaris x64 9 3 0
Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house <t>MATLAB</t> script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).
Imaris X64 9 3 0, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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data processing software  (Inscopix Inc)
90
Inscopix Inc data processing software
Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house <t>MATLAB</t> script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).
Data Processing Software, supplied by Inscopix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pls toolbox 8.7  (Eigenvector Research Inc)
90
Eigenvector Research Inc pls toolbox 8.7
Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house <t>MATLAB</t> script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).
Pls Toolbox 8.7, supplied by Eigenvector Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prism 8  (GraphPad Software Inc)
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GraphPad Software Inc prism 8
Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house <t>MATLAB</t> script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).
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Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house <t>MATLAB</t> script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).
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Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house <t>MATLAB</t> script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).
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Image Search Results


Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house MATLAB script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).

Journal: The New phytologist

Article Title: Chloroplast redox state changes mark cell-to-cell signaling in the hypersensitive response.

doi: 10.1111/nph.18425

Figure Lengend Snippet: Fig. 1 Chloroplast redox state is spatially regulated around the cell death zone during potato virus Y (PVY)-induced hypersensitive response (HR). (a) After inoculating leaves with PVY, the chloroplast redox state was mea- sured in transgenic sensor plants (pt-roGFP and salicylic acid (SA)-deficient pt-roGFP-NahG) with redox state-sensitive green fluorescent protein (roGFP) targeted to chloroplasts. The chloroplast redox state was mea- sured in three regions: the area adjacent to the lesion (region of interest 1, ROI1), the area adjacent to ROI1 (ROI2), and an area distant from the lesion (control, CTR) on the same leaf. As a negative control, the chloro- plast redox state was also measured in mock-inoculated plants (MOCK). To estimate the location of the lesion and determine the border between the cell death zone and normal tissue, the tissue was scanned in the chan- nel for the background chloroplast fluorescence detection (the border of the lesion is marked with mesophyll cells with disorganized chloroplasts that moved away from the cell periphery) and brightfield (to detect dead tissue). In normal tissue, chloroplasts are well arranged between the vac- uole and the cell wall, forming a ring in a circular shape of a mesophyll cell. (b, c) Visual presentation of the ratios of fluorescence intensities of roGFP after excitation with 405 and 488 nm laser (405 : 488 ratio, chloroplast redox state) presented on the rainbow scale for pt-roGFP L2 (b) and pt- roGFP-NahG L2 (c) in ROI1, ROI2 and MOCK (from left to right). Images are maximum projections from z-stacks processed with in-house MATLAB script. Higher ratios denote chloroplasts in a more oxidized state (red). Chloroplasts in the cells surrounding the cell death zone are highly oxi- dized, in contrast with more distant cells. Bar, 50 μm. (d, f) Chloroplast redox state in the earlier-mentioned leaf areas in pt-roGFP L2 (d) and pt- roGFP-NahG L2 (f) plants at 3, 5 or 7 d post-inoculation. Results are pre- sented as boxplots with 405 : 488 ratios of each measured ROI shown as dots (Exp3NahG and Exp5NT in Supporting Information Table S1). Gray lines connect ROI1 and ROI2 pairs for each lesion. Asterisks denote statisti- cally significant differences (P < 0.05) between the marked regions (ROI2, CTR or MOCK) and ROI1, determined by the mixed-effects model (ANOVA). Boundaries of the box, 25th and 75th percentiles; horizontal line, median; vertical line, all points except outliers. The experiment was per- formed three times independently on three transgenic lines (Redox Exp6NT in Fig. S1; Tables S1, S2; doi: 10.5281/zenodo.6417635). (e) Comparison of 405 : 488 ratios for marked regions (ROI1, ROI2, CTR and MOCK) between genotypes determined by the mixed-effects model (ANOVA). ns, not statistically significant. See Lukan et al. (2018, 2020) for the figures showing visual and microscopic lesion formation and reac- tive oxygen species (ROS) staining at different days post-inoculation (dpi).

Article Snippet: Image analysis was performed using an in-house MATLAB script (MATLAB and Image Processing Toolbox Release 2019b).

Techniques: Virus, Transgenic Assay, Control, Negative Control, Comparison, Staining